论文部分内容阅读
作为重要的丝孢类昆虫病原真菌,球孢白僵菌和玫烟色棒束孢因其易于生产和环境友好等优点而在害虫生防防治中受到广泛青睐。为初步探求孢子耐氧化力及其与孢子多糖含量的关系,球孢白僵菌和玫烟色棒束孢11株菌经胁迫后的残存指数随氧化剂H2O2浓度增加而减小。所有菌株的残存指数均能良好地与Logistic方程拟合,并计算出各菌株在氧化胁迫条件下的半致死浓度。结果显示玫烟色棒束孢孢子的耐氧化力强于球孢白僵菌。两种真菌的分生孢子耐氧力与各自多糖含量呈现良好线性正相关。培养基碳源成分和浓度变化可影响球孢白僵菌孢子耐氧化力,但耐氧化力与多糖含量依旧呈现线性正相关。由此可见,生防真菌分生孢子的耐氧化力的确与多糖积累有关,并在一定程度上受培养条件的调节。研究结果有望为提高生防真菌孢子环境稳定性提供新的策略。
As an important pathogenic fungus of the genus Sporozoites, Beauveria bassiana and Beauveria bassiana are widely favored in pest biocontrol because of their advantages of easy production and environmental friendliness. In order to explore the relationship between the spore oxidation resistance and the spore polysaccharide content, the residual index of 11 strains of Beauveria bassiana and B. obliquus decreased with the increase of H2O2 concentration. Residual indices of all strains were fitted well to Logistic equation, and the semi-lethal concentration of each strain under oxidative stress was calculated. The results showed that the resistance to oxidation of C. aeruginosa spores was stronger than that of B. bassiana. The conidial oxygen resistance of the two fungi showed a good linear correlation with their polysaccharides content. The composition and concentration of carbon source in culture medium can affect the spore oxidation resistance of B. bassiana, but the oxidation resistance and polysaccharide content still showed a linear positive correlation. Thus, biocontrol fungi conidia oxidation resistance is indeed related to the accumulation of polysaccharides, and to some extent by the adjustment of culture conditions. The results are expected to provide a new strategy to improve the environmental stability of biocontrol fungal spores.