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目的:分离莱姆病螺旋体。方法:鼠、蜱标本接种BSK培养基,分离病原体与单克隆抗体进行间接免疫荧光试验。结果:用BSK培养基分离49只野鼠和250匹蜱,从1只白腹巨鼠胚胎分离到1株螺旋体(LPR30)。结论:鉴定为莱姆病螺旋体
Objective: To isolate Lyme disease spirochetes. Methods: Rats and ticks were inoculated with BSK medium and indirect immunofluorescence assay was used to isolate pathogens and monoclonal antibodies. Results: 49 phyla and 250 ticks were isolated from BSK medium and 1 strain of spirochete (LPR30) was isolated from one white gazelle. Conclusion: It is identified as Lyme disease spirochete