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目的 探讨重组人促红细胞生成素(rHuEPO)对心梗大鼠心肌缺氧诱导因子-1α(HIF-1α)与生存素(Survivin)表达的影响.方法 45只SD大鼠,随机分为假手术组、心肌梗死组和rHuEPO治疗组.用Westernblot方法检测各组大鼠梗死区周围心肌中HIF-1 α与Survivin的表达水平,RT-PCR方法检测梗死区周围心肌HIF-1α mRNA与Survivin mRNA表达变化.结果 心肌梗死组大鼠心肌梗死区周围心肌HIF-1α蛋白与mRNA的表达水平显著高于假手术组,而Survivin蛋白与mRNA的表达水平则显著降低(P＜0.01);与心肌梗死组相比,rHuEPO治疗组心肌梗死区周围心肌HIF-1α蛋白与mRNA的表达水平明显降低,而Survivin蛋白与mRNA的表达水平则显著升高(P＜ 0.01).结论 rHuEPO能够抑制心梗大鼠心肌梗死区周围心肌HIF-1α过表达并上调Survivin的表达.“,”Objective To study the effect of recombinant human erythropoietin(rHuEPO) the expressions of HIF-1 α (Hypoxia-inducible factor-1 α) and Survivin in myocardial infarction rats.Methods 45 SD rats were randomly divided into the sham group,the myocardial infarction group(MI group) and recombinant human erythropoietin group(rHuEPO group).The expressions of HIF-1 α and Survivin proteins and mRNAs in parafarction area were examined by Western blot and RT-PCR respectively.Results The expression levels of HIF-1 α protein and mRNA in parafarction area were increased significantly in MI group than in sham group,but decreased in rHuEPO group than in MI group(P ＜ 0.01),The expression levels of Survivin protein and mRNA in parafarction area were decreased significantly in MI group than in sham group,but increased in rHuEPO group than in MI group(P ＜ 0.01).Conclusion rHuEPO could down-regulate the expression of HIF-1 α protein and mRNA and up-regulate the expression of Survivin protein and mRNA in parafarction area of myocardial infarction rats.