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目的建立用HPLC法测定栽培丹参中丹参素、丹酚酸B、原儿茶醛及咖啡酸含量的方法。方法用十八烷基键合硅胶为固定相Shim-packVP-ODS柱,以乙腈为流动相A,以0.026%磷酸水为流动相B,进行梯度洗脱,检测波长为280m。结果丹参素钠在0.1522～2.283μg范围内线性良好(r=0.9999),回收率为100.63%(RSD=2.04%,n=6)。丹酚酸B在0.2638～3.957μg范围内线性良好(r=0.9999),回收率为100.34%(RSD=1.58%,n=6)。原儿茶醛在0.02416～0.3624μg范围内线性良好(r=0.9999),回收率为99.74%(RSD=2.18%,n=6)。咖啡酸在0.04096～0.6144μg范围内线性良好(r=0.9999),回收率为99.17%(RSD=2.38%,n=6)。结论本方法简便,可同时测定栽培丹参中水溶性成分含量,结果准确。
Objective To establish a method for the determination of danshensu, salvianolic acid B, protocatechuic aldehyde and caffeic acid in cultivated Salvia miltiorrhiza by HPLC. Methods The octadecyl-bonded silica gel was used as the stationary phase Shim-pack VP-ODS column with acetonitrile as the mobile phase A and 0.026% phosphoric acid water as the mobile phase B. Gradient elution was carried out at a detection wavelength of 280 m. Results The calibration curve of Danshensu was linear in the range of 0.1522～2.283μg (r=0.9999). The recovery was 100.63% (RSD=2.04%, n=6). Salvianolic acid B was linear in the range of 0.2638-3.957 μg (r=0.9999), and the recovery was 100.34% (RSD=1.58%, n=6). The protocatechuic aldehyde was linear in the range of 0.02416-0.3624 μg (r=0.9999), and the recovery was 99.74% (RSD=2.18%, n=6). The linearity of caffeic acid was good in the range of 0.04096-0.6144 μg (r=0.9999), and the recovery was 99.17% (RSD=2.38%, n=6). Conclusion The method is simple and convenient, and the content of water-soluble components in cultivated Salvia miltiorrhiza can be determined at the same time. The result is accurate.