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目的:观察灯盏花乙素对脑缺血再灌损伤大鼠肝功能的影响。方法:大鼠灌胃给药7日后,阻塞大脑中动脉诱导脑缺血再灌损伤,检测大鼠血清和/或肝组织中的一氧化氮、黄嘌呤氧化酶、丙氨酸转氨酶、天冬氨酸转氨酶、丙二醛、抗氧化酶、及细胞色素P-450活性(水平)。结果:灯盏花乙素明显降低(P<0.01)血清中升高的黄嘌呤氧化酶、丙氨酸转氨酶、天冬氨酸转氨酶及肝细织中的丙二醛(P<0.05),升高血清(P<0.01)和肝组织(P<0.05)中降低的一氧化氮并可诱导超氧化物歧化酶(P<0.05)和谷胱甘肽过氧化物酶(P<0.01),除明显降低(P<0.01) CYP3A的活性外,大鼠脑缺血再灌损伤对肝CYP1A1、CYP1A2、CYP2E1的活性没有影响,灯盏花乙素不影响脑缺血再灌损伤大鼠肝CYP的活性。结论:大鼠脑缺血再灌可诱发肝细胞损伤,灯盏花乙素具有保护作用,其作用机制与抗氧化有关。
Objective: To observe the effect of scutellarin on liver function in rats with cerebral ischemia-reperfusion injury. METHODS: Seven days after intragastric administration, cerebral ischemic reperfusion injury was induced by occlusion of the middle cerebral artery. Nitric oxide, xanthine oxidase, alanine aminotransferase, and asparagine were detected in rat serum and/or liver tissues. Aminotransferase, malondialdehyde, antioxidant enzymes, and cytochrome P-450 activity (level). RESULTS: Scutellarin significantly decreased (P<0.01) elevated serum xanthine oxidase, alanine aminotransferase, aspartate aminotransferase, and malondialdehyde in liver fines (P<0.05). Serum nitric oxide (P<0.01) and liver tissue (P<0.05) could induce superoxide dismutase (P<0.05) and glutathione peroxidase (P<0.01), except for obvious The activity of CYP1A1, CYP1A2, and CYP2E1 in liver was not affected by CYP3A (P<0.01). Cerebral ischemia-reperfusion injury did not affect the activity of CYP in rats with cerebral ischemia-reperfusion injury. Conclusion: Cerebral ischemia and reperfusion can induce hepatocyte injury, and scutellarin has a protective effect, and its mechanism of action is related to antioxidation.