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流行性感冒（以下简称“流感”）的重要性是可引起严重的发病率和死亡率。流感疫苗的研究迄今年有50多年的历史，是目前唯一可行的流感预防措施。流感灭活疫苗生产二次收获的研究是尽最大的可能将一次收获后的鸡胚尿囊腔中残存的尿液、病毒络合物的沉淀、细胞内未释放的病毒和病毒残片二次收获回来。该方法可降低成本，提高效价，增加收获量。本实验根据渗透压原理，向第一次收获后的鸡胚尿囊腔中加入一定量的高渗液体，然后二次收获。小量试验三次，其结果为使用8．5％NaCl血凝效价为1：128，M/7．5PBS血凝效价为1：256，原液对照分别为1：100及1：128。根据小量结果大量收6批二次苗，其血凝效价可达到1：160以上，收率为第一次收获的70％左右，上述结果表明，流感灭活疫苗的二次收获是可行的。
The importance of influenza (hereinafter referred to as “flu”) can cause serious morbidity and mortality. The study of influenza vaccine has had more than 50 years of history so far and is the only practicable influenza preventive measure to date. The study on secondary harvest of influenza inactivated vaccine production is to try its best to make the residual urine and virus complex in the allantoic cavity of a harvested chicken embryo, the secondary harvest of un-released intracellular virus and virus fragments come back. This method can reduce costs, increase potency and increase yield. In this experiment, according to the principle of osmotic pressure, a certain amount of hypertonic liquid was added into the chick embryo allantoic cavity after the first harvest, and then harvested twice. A small amount of test three times, the results of the use of 8.5% NaCl blood coagulation titer of 1: 128, M / 7.5PBS hemagglutination titer of 1: 256, stock control were 1: 100 and 1: 128 respectively. According to a small amount of results received a large number of six batches of secondary seedlings, the blood coagulation titer can reach more than 1: 160, the yield of about 70% of the first harvest, the above results show that the second inactivated influenza vaccine is feasible of.