论文部分内容阅读
目的研究鱼藤酮所致的帕金森病大鼠的脑内α-突触核蛋白(α-synuclein, ASN)分布。方法 Wistar大鼠随机分成两组,分别给予鱼藤酮和/ 或溶剂(对照组)皮下注射,4 周后取脑组织,对黑质部位HE 染色,光镜下观察Lewy小体形态;对黑质、海马、纹状体等脑区进行酪氨酸羟化酶(tyrosine hydroxylase, TH)、ASN 免疫组织化学染色。结果在对照组大鼠脑内,ASN 广泛分布于各脑区,尤其在皮质、纹状体、海马等纤维投射丰富的区域。鱼藤酮处理的大鼠脑中,黑质TH阳性多巴胺能神经元数目减少、纹状体区TH阳性纤维脱失,黑质部位可见Lewy小体样结构;ASN阳性染色在各个脑区均有增强但各个脑区增强程度不一,黑质部位神经元胞浆和胞核内均有ASN明显聚集,纹状体可见ASN聚集围绕在细胞周围。海马部位偶见ASN在胞浆中点状聚集,胞核中无明显改变。结论在鱼藤酮皮下注射导致的帕金森病大鼠的脑内,ASN在多个脑区中表达增加,而在黑质纹状体部位聚集最为明显,蛋白分布由多巴胺能神经元的突触末端向胞浆和胞核扩展。
Objective To study the distribution of α-synuclein (ASN) in rotenone-induced Parkinson’s disease rats. Methods Wistar rats were randomly divided into two groups. The rats were injected subcutaneously with rotenone and / or solvent (control group), respectively. After 4 weeks, the brain tissue was taken and stained with hematoxylin and eosin for HE staining. Hippocampus, striatum and other brain regions tyrosine hydroxylase (tyrosine hydroxylase, THN, ASN immunohistochemical staining. Results In the control group, ASN was widely distributed in various brain regions, especially in areas with abundant fiber projection such as cortex, striatum and hippocampus. In the rotenone-treated rat brain, the number of TH-positive dopaminergic neurons in the substantia nigra was decreased, the TH-positive fibers in the striatum were lost, and the Lewy body-like structure was seen in the substantia nigra. ASN-positive staining was enhanced in all brain regions The enhancement of various brain regions was different. ASNs in the cytoplasm and nucleus of substantia nigra neurons were obviously aggregated. The striatum showed ASN clustered around the cells. Occasionally in the hippocampus ASN gathered in the cytoplasm dot-like, no significant change in the nucleus. Conclusions In the brain of Parkinson’s disease rats induced by subcutaneous injection of rotenone, the expression of ASN is increased in multiple brain regions, and most obviously in the nigrostriatal organs. The protein distribution is distributed from the synaptic terminals of dopaminergic neurons Cytoplasm and nucleus expansion.