论文部分内容阅读
传统的葡萄糖氧化酶电极基于下列催化反应:D—葡萄糖+O_2——→葡萄糖内脂+H_2O_2通过测定氧的消耗或生成的过氧化氩,可测得试液中葡萄糖含量。此种电极的缺点在于易受试样中溶氧的影响。而且因试样中溶氧量的限制,电极的线性上限小,一些试样需要稀释才能测定。Cass 以二茂铁衍生物吸附于石墨电极的表面,然后将葡萄糖氧化酶共价键合于石墨表面,制成第一个介体修饰型葡萄糖电极。该电极测定葡萄糖量不受底液
The traditional glucose oxidase electrode is based on the following catalytic reactions: D-glucose + O 2 - → glucose lactone + H 2 O 2 The glucose content in the test solution can be determined by measuring the oxygen consumption or the generated hydrogen peroxide. The disadvantage of such an electrode is that it is susceptible to dissolved oxygen in the sample. And because of the limited amount of dissolved oxygen in the sample, the upper limit of the electrode is small, and some samples require dilution to determine. Cass with ferrocene derivative adsorbed on the graphite electrode surface, and then glucose oxidase covalently bonded to the graphite surface, made of the first mediated glucose electrode. The electrode determines the amount of glucose from the bottom