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目的 :探讨诱导型一氧化氮合酶 (iNOS)在喉癌喉咽癌组织中的表达及其临床意义。方法 :采用逆转录 聚合酶链反应 (RT PCR)技术检测 4 0例喉癌及 12例喉咽癌 (喉癌喉咽癌组 )、5 0例癌旁组织 (癌旁组 )、12例良性肿瘤 (良性肿瘤组 )及 7例正常喉黏膜 (正常喉黏膜组 )组织中iNOSmRNA的表达。结果 :喉癌喉咽癌组阳性表达率为 78.85 % ,与正常喉黏膜组 (0 % )和癌旁组 (2 0 .0 0 % )比较 ,差异有极显著性意义 (P <0 .0 1) ;与良性肿瘤组 (4 1.6 7% )比较 ,差异亦有显著性意义 (P <0 .0 5 )。有局部淋巴结转移组 (95 .2 4 % )显著高于无局部淋巴结转移组 (6 7.74 % ) (P <0 .0 5 ) ;iNOSmRNA阳性表达与T分级呈正相关 (P <0 .0 5 ) ,与细胞分化程度呈负相关 (P <0 .0 5 )。结论 :喉癌喉咽癌组织中iNOSmRNA呈高表达 ,提示iNOS可能通过合成NO在分子水平参与了喉癌喉咽癌的发生、发展 ;NO供体药物和iNOS特异性抑制剂的开发 ,必将为肿瘤治疗带来可喜的前景。
Objective: To investigate the expression of inducible nitric oxide synthase (iNOS) in laryngeal carcinoma and its clinical significance. Methods: 40 cases of laryngeal carcinoma and 12 cases of hypopharyngeal carcinoma (laryngeal and hypopharyngeal carcinoma), 50 cases of paracancerous tissues (paracancer group) were detected by reverse transcriptase polymerase chain reaction (RT - PCR) (Benign tumor) and 7 cases of normal laryngeal mucosa (normal laryngeal mucosa) tissue iNOSmRNA expression. Results: The positive rate of laryngeal carcinoma in hypopharyngeal carcinoma was 78.85%, which was significantly different from that in normal laryngeal mucosa (0%) and paraneoplastic adjacent tissues (20.0%) (P <0. 0 1). Compared with benign tumor group (4 1.6 7%), the difference was also significant (P <0.05). There was a positive correlation between the positive expression of iNOS mRNA and T grade in patients with lymph node metastasis (95.24%) than those without lymph node metastasis (6 7.74%) (P <0.05) , But negatively correlated with cell differentiation (P <0.05). CONCLUSIONS: The high expression of iNOS mRNA in laryngeal carcinoma tissues suggests that iNOS may participate in the development and progression of laryngeal carcinoma hypopharyngeal carcinoma at the molecular level through the synthesis of NO. The development of NO donor drugs and iNOS-specific inhibitors will inevitably Bring promising prospects for cancer treatment.