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实验用30只成年栗鼠其中5只为对照组,13只暴露于155dB脉冲噪声、12只暴露于160 dB脉冲噪声,各50分钟。全部动物在经噪声暴露后45天处死,断头取颞骨。暴露噪声的两组动物中各有5只耳蜗用标准铺片法置光镜下观察。其余50只用Axelsson&Vertes“软铺片”技术处理后在相差显微镜下评价耳蜗血管状态。动物的耳蜗情况判定分为四类。(1)正常:耳蜗外毛细胞缺失率在2%以下;内毛细胞缺失率低于1%。(2)低度散在的毛细胞缺失:耳蜗外毛细胞缺失率在3～17%之间;内毛细胞缺失率在0～8%。(3)蜗中段灶性损伤:在1～5 KHz区域内耳蜗外毛细胞全部缺失;内毛细胞缺失10%以上。(4)重度损伤:从1 KHz区至基底转,耳蜗外毛细胞全部丧失,内毛细胞缺失
Thirty adult chinchillas were used as control in the experiment, 13 were exposed to 155dB impulsive noise and 12 were exposed to 160 dB impulsive noise for 50 minutes each. All animals were sacrificed 45 days after exposure to noise and decapitated temporal bone. Five cochleas in each of the two groups of animals exposed to noise were observed under a light microscope using a standard spreading method. The remaining 50 were treated with Axelsson & Vertes “Soft Tufted” technique to evaluate cochlear vascular status under a phase contrast microscope. Animal cochlear situation is divided into four categories. (1) normal: the loss rate of cochlear outer hair cells below 2%; the loss of inner hair cells less than 1%. (2) low scattered hair cells missing: the loss rate of cochlear outer hair cells in 3 to 17%; the loss of inner hair cells in 0 to 8%. (3) Focal injury in the middle of the cochlea: all outer hair cells of the cochlea are missing in the range of 1 ~ 5 KHz; the inner hair cells are missing more than 10%. (4) severe injury: from 1 KHz area to the basal turn, all the loss of outer hair cells, hair loss