影响奶牛人工授精成功率的主要原因是能否正确判断发情期,适时输精。Claus(1983)报告,由于输精时间不正确致使授精失败的牛占21.3％。根据乳汁中孕酮含量分析奶牛发情期的授精问题,国内未见报导,我们对此作了初步探索。乳汁孕酮含量测定主要根据Heap(1976)的放射免疫分析。 Affect the success rate of dairy artificial insemination, the main reason is whether the correct judgment of estrus, timely insemination. Claus (1983) reported that 21.3% failed to fertilize due to improper timing of insemination. According to the milk progestin content analysis estrus estrus question, there is no report, we made a preliminary exploration. Determination of milk progesterone content mainly based on Heap (1976) radioimmunoassay.