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严重干眼症是一种难治性眼表疾病,大多数患者因泪液缺乏,可导致角膜溃疡、混浊以至失明,此外稳定的泪膜对眼表重建后的上皮能否存活影响极大,如不采取积极措施改善干眼症、恢复泪膜,则眼表重建手术必将失败。近年来自体颌下腺移植为严重干眼症的治疗带来了希望,初步的临床研究证实进行腺体移植后,可重建泪膜,缓解干眼症状。但是作为一种新的术式,移植的颌下腺能否长期保持分泌功能,是否会发生萎缩,分泌成分是否会改变等问题尚无答案。本期论著《游离移植后长期存活的兔颌下腺细胞的生物学特性》(详见161页)的动物模型中,将移植成功后存活8个月的腺体细胞分离培养,通过流式细胞仪及MTT检测,证实了去除神经支配后游离移植的颌下腺细胞,生物学特性未发生明显的改变,具有与正常颌下腺细胞相似的生长特点,为开展自体
Severe dry eye disease is a refractory ocular surface disease, the majority of patients due to lack of tears, can lead to corneal ulceration, turbidity and even blindness, in addition to the stability of the ocular surface after reconstruction of the epithelium can have a great impact on survival, such as Do not take positive measures to improve dry eye, tear film repair, the ocular surface reconstruction surgery will fail. In recent years, autologous submandibular gland transplantation for the treatment of severe dry eye brought hope, preliminary clinical studies confirmed that after gland transplantation, reconstruction of tear film, ease the symptoms of dry eye. However, as a new surgical procedure, the submandibular gland transplantation can maintain the long-term secretion, whether there will be atrophy, secretion of the composition will change and so there is no answer. In this issue of the animal model of long-term survival of rabbit submandibular gland cells after free transplantation (see page 161 for details), glandular cells that survived for 8 months after transplantation were isolated and cultured by flow cytometry and MTT test confirmed that after removal of nerve cells after free transplantation submandibular gland cells, biological characteristics did not change significantly with the normal submandibular gland cells with similar growth characteristics for the development of autologous