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目的 研究大肠癌患者门静脉血中基因突变检测的可行性 ,探讨门静脉血微转移癌细胞与大肠癌肝转移间的关系。 方法 以聚合酶链反应 单链构象多态性分析 (PCR SSCP) /银染技术 ,检测 5 5例大肠癌患者门静脉血中p5 3和k ras基因的突变。 结果 5 5例患者门静脉血中检出基因突变阳性 2 2例 (4 0 % ) ,阳性检出与Dukes分期、肝转移显著相关。随访发现门静脉血基因突变阳性者 ,术后发生肝转移机会增加。 结论 门静脉血内微转移癌细胞的基因突变检测有助于判断大肠癌恶性程度和患者预后 ,对早期诊断或预测大肠癌肝脏微小转移有一定的临床价值
Objective To study the feasibility of detecting gene mutations in portal vein blood of patients with colorectal cancer and to explore the relationship between portal vein micrometastasis cancer cells and liver metastases of colorectal cancer. Methods Polymerase chain reaction single strand conformation polymorphism analysis (PCR-SSCP)/silver staining technique was used to detect p53 and k ras gene mutations in the portal vein blood of 55 patients with colorectal cancer. Results A total of 22 cases (40%) of positive mutations were detected in the portal vein blood of the 5 patients. Positive detection was associated with Dukes staging and liver metastasis. Follow-up found that portal vein blood gene mutation positive, postoperative liver metastasis increased. Conclusion Detection of gene mutations in portal vein blood micrometastatic cancer cells is helpful to determine the degree of malignancy and prognosis of patients with colorectal cancer. It has certain clinical value for early diagnosis or prediction of micrometastasis in colorectal cancer.