论文部分内容阅读
为了准确鉴定松茸,确保消费者品尝到货真价实的松茸,该研究基于SRAP(sequence-related amplified polymorphism分子标记,从300对引物组合中分别筛选出1对松茸特异性引物(ME10、EM6),并将这对引物扩增的特异条带进行克隆、测序和设计引物后,成功地构建了松茸特异性分子标记(304 bp)。松茸在特异性分子标记(304 bp)均能扩增出条带,该标记在分子水平上可准确、快速地鉴定出松茸,并可有效地保证松茸的品质。
In order to accurately identify matsutake and ensure that consumers can enjoy genuine matsutake, the study screened out one pair of matsutake specific primers (ME10 and EM6) from 300 primer combinations based on sequence-related amplified polymorphism (SRAP) After cloning, sequencing and designing primers, the specific molecular marker (304 bp) of matsutake was successfully constructed.And the specific molecular marker (matsutake) of matsutake was able to amplify the bands, The marker can accurately and quickly identify matsutake at the molecular level and can effectively ensure the quality of the matsutake.