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用４ｗｋ－ＳＥＡ、５ｗｋ－ＳＥＡ和６ｗｋ－ＳＥＡ加福氏佐剂，背部皮下注射免疫ＢＡＬＢ／ｃ小鼠，攻击感染后短期动态观察结果显示，４ｗｋ－ＳＥＡ免疫小鼠攻击感染后４～６ｗｋ时，小鼠肝组织内的减卵率（４０．７４％～５２．５９％）显著高于感染组和佐剂对照组（Ｐ＜０．０１），且在攻击感染后６ｗｋ时，免疫鼠肝组织内未见成熟虫卵，同期肝组织内肉芽肿平均直径（８１．５４±２０．７０μｍ～１１５．２８±２０．５２μｍ）明显小于对照鼠者（１５２．２２±６０．６９μｍ～３４９．１１±４８．４０μｍ），而用５ｗｋ－ＳＥＡ和６ｗｋ－ＳＥＡ免疫小鼠均未获得类似４ｗｋ－ＳＥＡ的免疫效应。研究结果表明，未成熟虫卵可溶性抗原能诱导出显著的抗病免疫力（明显的减卵率和成熟卵减少率及感染鼠肝内肉芽肿的显著缩小），具有理论意义和应用前景
BALB / c mice were immunized subcutaneously with 4wk-SEA, 5wk-SEA and 6wk-SEA plus Freund’s adjuvant. The short-term dynamic observations after challenge with 4wk-SEA showed that 4wk- The rate of oviposition in the liver of mice was significantly (40.74% -52.59%) higher than that of the infected and adjuvant control groups (P <0.01). At 6wk after challenge, No mature eggs were observed. The mean diameter of granulomas in liver tissue during the same period (81.54 ± 20.70μm ~ 115.28 ± 20.52μm) was significantly lower than that of control mice (152.22 ± 60.69μm ~ 349.11 ± 48 .40μm), while mice immunized with 5wk-SEA and 6wk-SEA did not achieve immune effect similar to 4wk-SEA. The results show that immature egg soluble antigen can induce significant resistance to immunity (significant reduction of the rate of ovulation and mature eggs and granulomas in mice infected liver significantly reduced), with theoretical significance and application prospects