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目的:建立加味藿香正气丸中百秋李醇、厚朴酚、和厚朴酚的GC含量测定方法。方法:色谱柱:Rtx-5柱(30m×0.32mm×0.5μm);以正十八烷为内标;柱温采用程序升温:起始150℃,保持23min,8℃/min升至230℃,保持35min;进样口温度:300℃;检测器(FID)温度:300℃;载气为氮气,流速为1.0ml·min~(-1);进样量1μl,分流比为20:1。结果:百秋李醇在0.082～0.82mg·ml~(-1)浓度范围内,与对照品峰面积与内标峰面积之比值呈良好的线性关系(r=0.9999),平均回收率为99.6%(RSD=1.5%,n=6);厚朴酚在0.504～5.04mg·ml~(-1)浓度范围内,与对照品峰面积与内标峰面积之比值呈良好的线性关系(r=0.9996),平均回收率为100.2%(RSD=1.4%,n=6);和厚朴酚在0.254～2.54mg·ml~(-1)浓度范围内,与对照品峰面积与内标峰面积之比值呈良好的线性关系(r=0.9998),平均回收率为100.4%(RSD=1.4%,n=6)结论:该方法简单、准确,可作为加味藿香正气丸的质量控制。
Objective: To establish a method for the determination of GC content of patchouli alcohol, honokiol and honokiol in Jiawei Huxiangzhengqi pills. Method: Column: Rtx-5 column (30m × 0.32mm × 0.5μm); n-octadecane as the internal standard; column temperature using a program temperature: the initial 150 °C, maintain 23min, 8 °C / min rose to 230 °C , Hold for 35 min; inlet temperature: 300°C; detector (FID) temperature: 300°C; carrier gas is nitrogen, flow rate 1.0 ml·min -1; sample volume 1 μl, split ratio 20:1 . Results: In the concentration range of 0.082～0.82mg·ml -1, there was a good linear relationship between the ratio of peak area and internal standard peak area (r=0.9999). The average recovery rate was 99.6. % (RSD=1.5%,n=6); the concentration of magnolol in the concentration range of 0.504～5.04mg·ml -1 showed a good linear relationship with the ratio of the peak area of the control to the peak area of the internal standard (r =0.9996), the average recovery was 100.2% (RSD = 1.4%, n = 6); the concentration of honokiol in the range of 0.254 - 2.54 mg · ml -1 and the peak area and internal standard peak of the reference substance The area ratio showed a good linear relationship (r=0.9998) and the average recovery was 100.4% (RSD=1.4%, n=6). Conclusion: This method is simple and accurate and can be used as a quality control for flavored Huxiangzhengqi pills.