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目的:制备蛇鳞草-大黄固体脂质纳米粒并考察其形态。方法:采用HPLC测定三羽新月蕨苷A和大黄素含量,流动相乙腈(A)-0.1%甲酸水溶液(B)-水(C)梯度洗脱(0~35 min,15%~75%A,25%B,60%~0%C),检测波长226 nm。利用薄膜超声法制备蛇鳞草-大黄固体脂质纳米粒,在单因素试验基础上,以三羽新月蕨苷A和大黄素包封率为综合评价指标,采用正交试验优选处方。结果:最佳处方为加药量150μL,硬脂酸-卵磷脂质量比1∶2,吐温-80质量分数1%;纳米粒平均粒径(301±2.8)nm,Zeta电位(-19.94±3.82)mV,三羽新月蕨苷A和大黄素包封率分别为61.8%和81.2%。结论:制备的蛇鳞草-大黄固体脂质纳米粒基本呈规则球形或类球形,该工艺稳定可行。
OBJECTIVE: To prepare snakehead-rhubarb solid lipid nanoparticles and investigate their morphology. Methods: HPLC was used to determine the contents of three new cinnabarinoside A and emodin. The mobile phases were eluted with a gradient of acetonitrile (A) - 0.1% formic acid (B) - water (C) A, 25% B, 60% ~ 0% C) at a detection wavelength of 226 nm. On the basis of the single factor experiment, the entrapment efficiency of three crescent tropinoside A and emodin was taken as the comprehensive evaluation index, and the prescription was optimized by orthogonal test. Results: The optimal dosage was 150μL, the mass ratio of stearic acid to lecithin was 1: 2 and the mass fraction of Tween-80 was 1%. The mean diameter of nanoparticles was (301 ± 2.8) nm and the Zeta potential was -19.94 ± 3.82) mV. The entrapment efficiencies of three crescent tropinoside A and emodin were 61.8% and 81.2%, respectively. Conclusion: The preparation of snake grass - rhubarb solid lipid nanoparticles basically spherical or spherical regularly, the process is stable and feasible.