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背景与目的肺癌转移相关基因-1(Pulmonary cancer Metastasis Associated Gene-1,MAG-1)是利用抑制消减杂交技术获得的与肺癌转移相关的基因。此研究的目的在于探讨基因MAG-1对肺巨细胞癌细胞的转移相关生物学行为的影响及其在24例肺癌组织中的表达情况。方法构建MAG-1的正反义表达载体,分别转染PLA801C和PLA801D,获得稳定表达相应序列的细胞株,观察稳定转染细胞的多种肿瘤转移相关能力的改变,并检测MAG-1对CD44、MMP-2等分子的影响,最后还检测了MAG-1在源于不同转移状况患者的肺癌标本中的表达情况。结果MAG-1对细胞生长、细胞与胞外基质的粘附能力以及侵袭能力均有促进作用;并能促进细胞中CD44和MMP-2的表达;MAG-1mRNA在有转移的肺癌中的阳性率(7/12)高于其在无转移的肺癌标本中的阳性率(2/12)。结论MAG-1具有增强肺癌细胞转移能力的功能,并且在转移性肺癌组织中其mRNA具有较高的阳性检出率,是可能的肺癌转移相关基因。
Background and Objective Pulmonary cancer Metastasis Associated Gene-1 (MAG-1) is a gene related to lung cancer metastasis obtained by suppression subtractive hybridization. The purpose of this study was to investigate the effect of gene MAG-1 on the metastasis-related biological behaviors of lung giant cell carcinoma cells and its expression in 24 lung cancer tissues. Methods The antisense and antisense expression vectors of MAG-1 were constructed and transfected into PLA801C and PLA801D respectively to obtain stable cell lines stably expressing the corresponding sequences. The changes of metastatic ability of various metastatic cells were observed. The effect of MAG-1 on CD44 , MMP-2 and other molecules, and finally detected the expression of MAG-1 in lung cancer specimens from patients with different metastatic status. Results MAG-1 promoted the cell growth, cell adhesion and invasion to cells and extracellular matrix, and promoted the expression of CD44 and MMP-2. The positive rate of MAG-1 mRNA in metastatic lung cancer (7/12) was higher than that in non-metastatic lung cancer specimens (2/12). Conclusions MAG-1 has the function of enhancing the metastasis of lung cancer cells, and its mRNA has a high positive rate in metastatic lung cancer tissues, which is a possible lung metastasis-related gene.