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目的:探讨辅助生殖技术(ART)与早期流产胚胎染色体数目异常的关系。方法:通过多重连接依赖探针扩增技术对145例ART妊娠和36例自然妊娠孕早期自然流产的绒毛组织进行染色体数目检测,分析流产胚胎的染色体异常情况,比较不同受孕方式、受精方式、移植新鲜/冷冻胚胎妊娠后自然流产绒毛染色体异常率。结果:所有早期自然流产绒毛标本染色体总异常率为66.85%(121/181),以常染色体三体为主,非整倍体涉及除1号染色体以外所有染色体,以16号染色体最多见。流产胚胎非整倍体发生率在自然妊娠组与ART组间(66.67%vs 66.90%,P=1.000)、新鲜胚胎移植组与冷冻胚胎移植组间(65.91%vs 68.42%,P=0.857)均无统计学差异,体外受精(IVF)组略高于卵胞质内单精子注射(ICSI)组,但无统计学差异(73.26%vs 57.63%,P=0.072)。结论:胚胎染色体数目异常是自然流产的主要病因,ART包括ICSI和冻融胚胎均没有增加胎儿非整倍体发生率。
Objective: To explore the relationship between assisted reproductive technology (ART) and the number of abnormal chromosomes in early abortion embryos. Methods: The number of chromosomes was detected in 145 cases of ART pregnancy and 36 cases of spontaneous abortion in the first trimester of pregnancy by multiplex ligation-dependent probe amplification. Chromosome abnormalities of aborted embryos were analyzed, and different methods of pregnancy, fertilization, transplantation Abnormal rate of spontaneous abortion chorion after fresh / frozen embryo pregnancy. Results: The total chromosome aberration rate of all abortion specimens was 66.85% (121/181). Aneuploidy was the most common one. Aneuploidy involved all chromosomes except chromosome 1, most notably chromosome 16. The incidence of aneuploidy in abortion embryos was between normal pregnancy group and ART group (66.67% vs 66.90%, P = 1.000), between fresh embryo transfer group and frozen embryo transfer group (65.91% vs 68.42%, P = 0.857) There was no significant difference between IVF group and ICSI group, but there was no significant difference (73.26% vs 57.63%, P = 0.072). CONCLUSION: Abnormal embryonic chromosome number is the main cause of spontaneous abortion. ART, including ICSI and frozen-thawed embryos, did not increase the incidence of fetal aneuploidy.