百草枯(1,1′-二甲基-4,4′-联吡啶离子盐)是一种常用的除草剂,通常采用薄层层析、高压液相色谱、紫外光谱、极谱等法测定。本文介绍一种灵敏的分光光度法测定水、谷类、植物中的百草枯。在碱性条件下,百草枯被抗坏血酸还原,产生一种蓝色基团离子,在波长600nm处有最大吸收。在10ml溶液中,百草枯含量在12～96μg(1.2～9.6ppm)之间符合 Paraquat (1,1’-dimethyl-4,4’-bipyridinium ion salt) is a commonly used herbicide, usually determined by thin-layer chromatography, high pressure liquid chromatography, UV spectroscopy, polarography and other methods . This article describes a sensitive spectrophotometric method for the determination of paraquat in water, grains and plants. Under alkaline conditions, paraquat is reduced by ascorbate, producing a blue group ion with maximum absorption at a wavelength of 600 nm. Paraquat levels ranged from 12 to 96 μg (1.2 to 9.6 ppm) in 10 ml of solution