The Three Gorges Reservoir(TGR) is suffering from unexpected fine sediment deposition,to better understand the fine sediment transport processes,field measurements were conducted at the Zhongxian and Fengjie reaches.A method based on the sediment diffusion equation was proposed to measure the settling velocities using the Acoustic Doppler Velocimeter(ADV).The backscatter acoustic intensities(BSI) received from the ADV were calibrated against the sediment concentrations measured via water sampling,suggesting a linear relationship in double logarithmic coordinate system.The instantaneous sediment concentration was calculated using the derived relationship,and then the settling velocity was obtained through the proposed procedure.The settling velocities of the fine particles in the TGR were found to vary with the water depth.Most of the effective settling velocities were within the range of 0.1-10 mm/s,which were larger than those of the primary particles,indicating that the flocculation was likely to occur in the TGR.Additionally,it is suggested that the turbulent motion played an important role in the flocculation in the TGR. The Three Gorges Reservoir (TGR) is suffering from unexpected fine sediment deposition, to better understand the fine sediment transport processes, field measurements were conducted at the Zhongxian and Fengjie reaches. A method based on the sediment diffusion equation was proposed to measure the settling velocities using the Acoustic Doppler Velocimeter (ADV). The backscatter acoustic intensities (BSI) received from the ADV were calibrated against the sediment concentrations measured via water sampling, suggesting a linear relationship in double logarithmic coordinate system. The instantaneous sediment concentration was calculated using the derived relationship, and then the settling velocity was obtained through the proposed procedure. The settling velocities of the fine particles in the TGR were found to vary with the water depth. Host of the effective settling velocities were within the range of 0.1-10 mm / s , which were larger than those of the primary particles, indicating that the flocculation was likely to occur in the TGR.Additionally, it is suggested that the turbulent motion played an important role in the flocculation in the TGR.