OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis. OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum. METHODS We isolated total protein from cell lysis solutions and cultured supernatants from 2 human ovarian cancer cell lines and used SELDI-TOF-MS to detect the differential expression of the proteins in the 2 cell lines. proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients, relapsed ovarian cancer patients, patients withbenign ovarian tumors, and healthy people .RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated, relative to the cultures of supernatant of HO-8910 cells. One protein (3,144 Da m / z value) was up-regulated in both cell lysis solution and in the cultures of supernatant of HO- of the 3,144 Da m / z protein differed significantly between serum from the 26 ovarian cancer patients, from the 22 relapsed ovarian patients and There was no difference between patients with benign ovarian tumors and healthy people (P> 0.5) .CONCLUSION Ovarian cancer cell lines with high or low metastatic potential have distinct protein profiles. Protein 3,144 Da m / z could be a useful biomarker for diagnosing ovarian cancer metastasis.