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背景:基质蛋白是构成血管壁的必不可少的重要成分,为血管壁的完整性和血管壁细胞发挥生理功能提供了必要的框架,并参与对细胞和平滑肌的调控。目的:构建早期动脉瘤模型大鼠评价基质结构蛋白在形成过程中的表达差异性。方法:将28只健康雄性SD大鼠随机分为2组:对照组(n=8)和模型组(n=20)。模型组大鼠以结扎左侧颈总动脉和右侧肾肾动脉致高血压方法建立脑动脉瘤模型;对照组大鼠仅暴露左侧颈动脉分叉和双侧颈动脉。模型组大鼠分别于造模后15,30 d处死,取大鼠右侧大脑前动脉和嗅动脉的分叉处部分组织进行免疫组化染色,分析纤维连接蛋白、α-平滑肌肌动蛋白和Ⅲ型胶原蛋白的表达。结果与结论:与对照组相比,造模后30 d时模型组大鼠右侧大脑前动脉和嗅动脉的分叉处部分中纤维连接蛋白表达水平差异无显著性意义(P>0.05),而Ⅲ型胶原和α-平滑肌肌动蛋白表达明显减少(P<0.05)。提示大鼠早期脑动脉瘤形成过程中的结构蛋白表达存在差异性并发生动态变化,血管壁基质结构蛋白降解是动脉瘤形成主要机制之一。
BACKGROUND: Matrix proteins are essential components of the vascular wall, providing the necessary framework for the integrity of the vascular wall and the physiological functions of vascular parietal cells, and are involved in the regulation of cells and smooth muscle. OBJECTIVE: To construct early aneurysm model rats to evaluate the differences in the expression of stromal structural proteins in the process of their formation. Methods: 28 healthy male SD rats were randomly divided into two groups: control group (n = 8) and model group (n = 20). In the model group, the model of cerebral aneurysm was established by ligation of the left common carotid artery and the right renal artery and renal hypertension. In the control group, only the left carotid bifurcation and bilateral carotid artery were exposed. Rats in the model group were sacrificed at 15 and 30 days after modeling, and the tissues of the right anterior cerebral artery and olfactory artery at the bifurcation were harvested for immunohistochemical staining. The expression of fibronectin, α-smooth muscle actin and Type Ⅲ collagen expression. RESULTS AND CONCLUSION: Compared with the control group, there was no significant difference in the expression of fibronectin between the anterior cerebral artery and olfactory artery at the 30th day after model establishment in the model group (P> 0.05) While the expression of type Ⅲ collagen and α-smooth muscle actin decreased significantly (P <0.05). It suggested that the structural protein expression during early cerebral aneurysm formation in rats is different and dynamically changed. The degradation of vascular matrix protein is one of the main mechanisms of aneurysm formation.