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目的：制备从尖吻蝮蛇蛇毒中分离的纤溶因子的单克隆抗体，为克隆基因提供特异性的探针。方法：将纤溶因子免疫Ｂａｌｂ／Ｃ小鼠，取脾细胞与ＳＰ２／０骨髓瘤细胞融合，筛选稳定分泌抗体的细胞株。采用血纤维蛋白平板法，观察单克隆抗体能否抑制纤溶因子的纤溶作用。结果：获得２株稳定分泌单克隆抗体的细胞株。Ｗｅｓｔｅｒｎ印迹显示该单克隆抗体能特异地与纤溶因子结合，与其它蛋白无交叉反应，此外，这２株单克隆抗体具有抑制纤溶因子溶解人血纤维蛋白的作用。结论：这２株抗体不仅能特异地与纤溶因子结合，而且具有抑制纤溶的作用，为克隆纤溶因子基因及其功能表达提供了特异性的研究工具。
OBJECTIVE: To prepare a monoclonal antibody to fibrinolytic factor isolated from Agkistrodon acutus Venom, and to provide a probe for cloning genes. Methods: Balb / C mice were immunized with fibrinolytic factor. The spleen cells were fused with SP2 / 0 myeloma cells to screen stable cell lines secreting antibodies. Using fibrin plate method, observe the monoclonal antibody can inhibit the fibrinolytic factor fibrinolysis. Results: Two cell lines stably secreting monoclonal antibodies were obtained. Western blotting showed that the monoclonal antibody specifically binds to fibrinolysis without cross-reaction with other proteins. In addition, these two monoclonal antibodies have the effect of inhibiting fibrinolysis of human fibrin. CONCLUSION: These two antibodies can not only bind fibrinolytic factor specifically but also inhibit the fibrinolysis, providing a specific research tool for cloning fibrinolysis genes and their functional expression.