精子疫苗作为免疫避孕的方法之一正日益引起人们的重视,ELISA法作为检测不育患者体内抗精子抗体的方法也不断得到推广和普及,其前提之一都是要求获得大量有效的精子抗原。传统的用物理或化学方法直接从精子中提取有限的抗原已远不能满足需要。 As one of the methods of immunization contraception, sperm vaccine is attracting more and more attention. As a method to detect anti-sperm antibodies in infertile patients, ELISA method has been popularized and popularized continuously. One of the preconditions is to obtain a large number of effective sperm antigens. The traditional physical or chemical methods directly extracted from sperm limited antigen is far from meeting the needs.