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本文作者进行了羊垂体和大脑的不同部位在外分泌免疫活性甲状旁腺激素(PTH)的研究 材料和方法:处死动物后,立刻取出脑回(中枢前和中枢后)、内囊、基底神经节、小脑以及垂体。剥离组织,取各个部位及垂体标本(0.5g)。切成大约1mm的薄片)培育在含有10ml培养基的烧瓶内。后者为无血清的Hank’s溶液,37℃、95％空气和5％CO_2,以及低浓度Ca(0.7mM)中,放置6hr以上。
The authors performed a study of the exocrine immunologically active parathyroid hormone (PTH) in different parts of the goat and brain. MATERIALS AND METHODS: Immediately after the animals were sacrificed, the brainstem (anterior and central), internal capsule, basal ganglia , Cerebellum and pituitary gland. Peel the tissue, take all parts and pituitary specimens (0.5g). Cut into pieces of about 1 mm) were incubated in a flask containing 10 ml of medium. The latter was placed in serum-free Hank’s solution for more than 6 hours at 37 ° C, 95% air and 5% CO 2, and low concentrations of Ca (0.7 mM).