论文部分内容阅读
超薄切片观察云南烟草丛枝症病害的病叶组织，并抽提试验材料的总核酸电泳检测，结果表明：病组织中检测到与烟草丛枝症病害相关的低分子量ＲＮＡ，切片中未观察到病毒粒子，薄壁细胞中发现细胞膜系统的畸变：质膜体（ｐｌａｍａｌｅｍｍａｓｏｍｅ）和双层膜泡囊（ｄｏｕｂｌｅｍｅｍｂｒａｎｏｕｓｖｅｓｉｃｌｅｓ）．质膜体由细胞质膜或液泡膜伸入空泡形成，直径约１０００～２０００ｎｍ，结构类似于类病毒侵染寄主形成的旁壁体．双层膜泡囊是含纤维状物内含物的泡囊，直径约１００～２００ｎｍ，结构类似于某些病毒侵染寄主形成的细胞质泡囊（ｃｙｔｏｐｌａｓｍｉｃｖｅｓｉｃｌｅｓ）和植原体（ｐｈｙｔｏｐｌａｓｍａｓ），但因其广泛分布于寄主细胞中，不能认为是植原体．文献显示这些病变是类病毒和某些病毒侵染的特征，初步认为云南烟草丛枝症病害可能是病毒病害，发病早期细胞内出现小分子核酸侵染的细胞病变特征，此与莫笑晗等发现病株体内含有大量低分子量ＲＮＡ可能有密切关系．
Ultrathin sections were observed in diseased leaves of tobacco witches’ bush disease in Yunnan Province. Total RNA was extracted from the tested materials. The results showed that low molecular weight RNAs were detected in the diseased tissues and were not observed in the sections To virus particles, parenchymal cells were found to have aberrations in the cell membrane system: plamalemmasome and doublemembranous vesicles. Plasma membrane from the plasma membrane or vacuolar membrane into the formation of vacuoles, the diameter of about 1000 ~ 2000nm, the structure is similar to the virus-like virus-infected host formation of the side wall. Bilayer vesicles are vesicles containing fibrous inclusions approximately 100-200 nm in diameter and structurally similar to certain viruses that infect host-forming cytoplasmic vesicles and phytoplasmas, but because of their Widely distributed in the host cells, can not be considered phytoplasma. The literature shows that these lesions are characteristic of the virus and certain virus infection. It is preliminarily believed that the disease may be a viral disease in Yunnan tobacco and the cytopathic feature of small molecule nucleic acid infection in the early onset of disease. Found that diseased plants contain large amounts of low molecular weight RNA may be closely related.