巴丹、易卫杀在大鼠胃匀浆中生物转化的研究

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建立了胃匀浆中巴丹、易卫杀的GC-FPD测定方法,并用此方法观察了巴丹、易卫杀在大鼠胃匀浆中的转化。添加巴丹的胃匀浆样品,经Na2S溶液转化生成沙蚕毒素,其回收率为103.1%[n=4,RSD(%)=0.73];添加易卫杀的胃匀浆样品,其回收率为100.5%[n=4,RSD(%)=0.54]。在大鼠胃匀浆中分别添加巴丹、易卫杀后,1min时,分别有49.7%和28.8%的巴丹、易卫杀转化成沙蚕毒素;至10min时,加入的巴丹及易卫杀大部分转化生成沙蚕毒素。结果显示,巴丹和易卫杀在大鼠体内极易转化成沙蚕毒素。 Gastric homogenate in the establishment of Badan, easy to kill GC-FPD determination method, and the use of this method to observe the Badan, easy to kill in the rat stomach homogenate conversion. The gastric homogenate sample of Bardan was added and the neotoxin was converted into Na2S solution with a recovery of 103.1% [n = 4, RSD (%) = 0.73] The recovery was 100.5% [n = 4, RSD (%) = 0.54]. In the gastric homogenate of rats were added Batian, easy to kill, 1min, respectively, 49.7% and 28.8% of Bataan, easy to kill into Nereis toxin; to 10min, added Badan and easy to kill most of the conversion of Nereis toxin. The results show that Badan and easy to kill easily transformed into Nereis in rats.
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