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反胶团萃取法是一种新型、高效的生物活性物质分离技术。通过五因素三水平的正交试验,研究反胶团体系中丁二酸-2-乙基己基酯磺酸钠(AOT)浓度、萃取pH、萃取离子强度、反萃取pH、反萃取离子强度对柚皮苷提取的影响。结果表明,萃取pH值和反萃取pH值对柚皮苷的反胶团萃取起着显著作用,因素作用的主次顺序为萃取pH值>反萃取pH值>萃取离子强度>AOT浓度>反萃取离子强度。最优提取条件为萃取pH4.0、反萃取pH10.0、萃取离子强度0.2mol/L、AOT浓度50.0mmol/L、反萃取离子强度0.5mol/L。柚皮苷的萃取率可达96.15%。
Inverse micellar extraction is a new and efficient bioactive material separation technology. Through the orthogonal experiment of five factors and three levels, the concentration of AOT, the pH value, the intensity of extraction, the pH of the extract, the intensity of the stripping ion were studied in the reverse micelle system Effect of naringin extraction. The results showed that extraction pH value and back-extraction pH value played a significant role in reverse micellar extraction of naringin. The order of the main factors was the extraction pH value> pH value of extraction> extraction ionic strength> AOT concentration> Ionic strength. The optimal extraction conditions were pH 4.0, pH10.0, extraction concentration 0.2mol / L, AOT concentration 50.0mmol / L, and stripping ionic strength 0.5mol / L. Naringin extraction rate of up to 96.15%.