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目的:研究在固定时间和频率下,三角波形的低频脉冲磁场(LF-PMF)对大鼠心肌微血管内皮细胞(CMECs)增殖、迁移以及细胞周期的影响。方法:实验分为4组(对照组,1.0mT组,1.4mT组,1.8mT组)。对照组不加磁场干预;其余各组分别在频率为15 Hz,磁场强度分别为1.0mT、1.4mT和1.8mT,时间为4 h/d,连续照射3 d的条件下,用三角波形作用离体培养的大鼠CMECs。利用MTT法检测细胞增殖情况,transwell法观察细胞迁移状况,流式细胞仪技术检测细胞周期变化。结果:1.4 mT和1.8 mT组磁场促进CMECs增殖的作用差异显著[(0.200±0.043)A值,vs.(0.159±0.037)A值,(0.225±0.042)A值,vs.(0.159±0.037)A值,P<0.05]。迁移能力与对照组相比也有显著提高[(27.20±4.76)个/视野vs.(22.60±4.77)个/视野,(33.80±3.19)个/视野vs.(22.60±4.77)个/视野,P<0.05]。CMECs在细胞周期中的分布发生改变,DNA合成期(S期)和合成后期(G2期)细胞的比例增加。1.0mT组LF-PMF胞对细胞增殖、迁移以及细胞周期的影响均不明显。结论:低频脉冲磁场磁场对CMECs的生物学作用与磁场的强度相关,1.4mT和1.8mT组LF-PMF促进细胞增殖、迁移及使其DNA合成的能力提高。
Objective: To study the effects of LF-PMF on the proliferation, migration and cell cycle of rat cardiac microvascular endothelial cells (CMECs) under fixed time and frequency. Methods: The experiment was divided into 4 groups (control group, 1.0mT group, 1.4mT group, 1.8mT group). The control group without magnetic field intervention; the rest of the group were at a frequency of 15 Hz, the magnetic field strengths were 1.0mT, 1.4mT and 1.8mT, the time of 4 h / d, continuous irradiation 3 d conditions, Cultured rat CMECs. The cell proliferation was detected by MTT assay. The cell migration was observed by transwell assay. The cell cycle was detected by flow cytometry. Results: The effects of magnetic fields of 1.4 mT and 1.8 mT on the proliferation of CMECs were significantly different (A> 0.200 ± 0.043 A vs 0.159 ± 0.037 A, 0.225 ± 0.042 A vs 0.159 ± 0.037, A value, P <0.05]. Migration capacity was also significantly improved compared with the control group [(27.20 ± 4.76) / field vs. (22.60 ± 4.77) / field, (33.80 ± 3.19) field / field vs. (22.60 ± 4.77) field / field, P <0.05]. The distribution of CMECs in the cell cycle changes, and the proportion of cells in the DNA synthesis phase (S phase) and the synthesis phase (G2 phase) increases. LF-PMF cells in 1.0mT group had no obvious effect on cell proliferation, migration and cell cycle. CONCLUSION: The biological effects of low frequency pulsed magnetic field on CMECs are related to the strength of magnetic field. The ability of LF-PMF in 1.4mT and 1.8mT groups to promote cell proliferation, migration and DNA synthesis was improved.