家兔子宫活动慢性记录方法的研究

来源 :中医药学报 | 被引量 : 0次 | 上传用户:cstmddn
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为了在慢性实验的条件下记录家兔子宫的活动,我们应用霍耳效应原理,可以准确的记录家兔子宫收缩的强度变化和频率变化。实验前给动物先做手术,手术过程中在子宫角埋藏磁块,缝合电极和安装子宫角固定器。埋藏在子宫角上的磁块随子宫活动变动位置,这就引起霍耳探头定点的磁场强度相应的变化从而导致霍耳电压的变化。霍耳电压的微弱变化经放大后记录在图纸上。为了判断这种方法的可靠性,我们同时记录子宫收缩的张力变位曲线和子宫电位曲线可清楚的看到它们之间有相关性变化。给动物注射催产素时,可记录到,子宫收缩明显增强的曲线,这说明应用霍耳效应方法所反映的子宫活动的变化符合这种药对子宫平滑肌的药理作用。这种方法能消除多种干扰因素。它能防止腹肌收缩对记录的影响;能防止呼吸运动对记录的影响;也能防止肠管运动对子宫活动影响。 In order to record the activity of the rabbit house in chronic experimental conditions, we apply the Hall effect principle to accurately record the changes in intensity and frequency of contractions in rabbits. Animals were subjected to surgery before the experiment. Magnetic blocks were buried in the uterine horn during surgery, and electrodes and sutured uterine horn holders were attached. The magnetic block buried in the uterine horn changes position with the uterine activity, which causes a corresponding change in the magnetic field intensity at the fixed point of the Hall probe, which results in a change in the Hall voltage. The small change in Hall voltage is amplified and recorded on the drawing. In order to judge the reliability of this method, we recorded the tension displacement curve and uterine potential curve of uterine contractions at the same time. We can clearly see that there are correlation changes between them. When oxytocin was injected into animals, a marked increase in uterine contraction was recorded, indicating that the change in uterine activity as reflected by the application of the Hall effect method was consistent with the pharmacological effects of this drug on uterine smooth muscle. This method can eliminate a variety of interference factors. It can prevent the impact of abdominal contraction on the recording; it can prevent the influence of respiratory movement on the recording; it can also prevent the effect of intestinal motion on uterine activity.
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