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目的:对18例复发性流产患者采用主动免疫方法,治疗前后进行CD4+CD25+highT细胞、CD4+T细胞、CD8+T细胞水平检测,探讨T细胞群在妊娠期参与调控母体免疫反应中的作用。方法:采用流式细胞仪分别对18例复发性流产患者在流产后初诊时、免疫治疗后3次、孕后免疫治疗2次时其外周血淋巴细胞CD4+CD25+highT细胞、CD4+T细胞、CD8+T比例进行分析。结果:18例复发性流产患者的CD8+T细胞占淋巴细胞初诊时比例为(22.5±1.35)%、免疫治疗后3次比例为(26.10±1.62)%;CD4+CD25+highT细胞占淋巴细胞比例初诊时为(0.98±0.26)%,免疫治疗后3次比例为(1.25±0.35)%;其中12例复发性流产患者孕后免疫治疗2次时的CD8+T占淋巴细胞比例为(29.50±2.20)%,CD4+CD25+highT细胞占淋巴细胞比例为(1.56±0.29)%。结论:主动免疫治疗可以提高外周血CD4+CD25+highT、CD8+T细胞水平,从而调控母胎界面免疫耐受的形成。
OBJECTIVE: To detect the levels of CD4 + CD25 + high T cells, CD4 + T cells and CD8 + T cells before and after treatment in 18 patients with recurrent spontaneous abortion to explore the role of T cell population in the regulation of maternal immune response during pregnancy effect. Methods: Flow cytometry was used to detect the percentage of CD4 + CD25 + high T cells and CD4 + T cells in peripheral blood mononuclear cells of 18 patients with recurrent spontaneous abortion when they were newly diagnosed after abortion, 3 times after immunotherapy and 2 times after immunotherapy. , CD8 + T ratio analysis. Results: The percentage of CD8 + T cells in 18 patients with recurrent spontaneous abortion was (22.5 ± 1.35)% when lymphocytes were newly diagnosed, and (3.10 ± 1.62)% after immunotherapy. The percentage of CD4 + CD25 + high T cells in lymphocytes (0.98 ± 0.26)% at first visit, and (1.25 ± 0.35)% at 3 times after immunotherapy. The proportion of CD8 + T to lymphocytes in 12 patients with recurrent spontaneous abortion after second immunization was 29.50 ± 2.20)%, the ratio of CD4 + CD25 + high T cells to lymphocytes was (1.56 ± 0.29)%. Conclusion: Active immunotherapy can improve the level of CD4 + CD25 + high T and CD8 + T cells in peripheral blood and regulate the immune tolerance of maternal-fetal interface.