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目的：为了进一步了解肺癌细胞生物学行为，评价肺小标本ＩＣＭ—ＤＮＡ含量检测对肺癌的诊断价值。方法：选取纤维支气管镜活检肺小标本 ９５例，其中肺癌组 ６４例［包括鳞状上皮细胞癌（简称鳞癌）４５例，腺癌 ９例，小细胞未分化癌１０例］，肺良性病变（炎症）组３１例。将肺小标本石蜡包块以５μｍ连续切片，分别作ＨＥ染色和Ｆｅｕｌｇｅｎ 染色，后者进而用国际标准化 ＣＡＳ－２００型 图像细胞仪（ＩＣＭ）检测 ＤＮＡ含量。分析指标包括 ＤＮＡ质量、ＤＮＡ指数（ＤＩ）、增殖指数（ＰＩ）、Ｓ期百分比、核面积、直方图类型及倍体状况等。结果：肺癌组细胞核ＤＮＡ质量、ＤＩ、ＰＩ、Ｓ％和核面积等指标均明显高于肺炎症组（Ｐ＜０．０５或Ｐ＜０．０１）；肺癌组ＤＮＡ直方图类型主要为Ⅲ型和Ⅵ型（占７９．７％），未见Ⅰ型；而肺炎症组相反，无１例为Ⅲ型和Ⅵ型，多呈Ⅰ型改变（９０．３％），两组之间有显著差异（Ｐ＜０．０１）；两组的细胞核 ＤＮＡ 倍体状况存在极显著差异（Ｐ＜０．０１），肺癌组多为异倍体，异倍体检出率达９６．９％，而肺炎症组均为二倍体。以异倍体为标准，结合Ｓ期百分比，诊断肺癌的敏感性为９６．７％，特异性达１００％。结论：应用ＩＣＭ检测纤维支气管?
Objective: To further understand the biological behavior of lung cancer cells, to evaluate the diagnostic value of small lung specimen ICM-DNA assay for lung cancer. METHODS: A total of 95 lung specimens were examined by fiberoptic bronchoscopy, including 64 lung cancer patients (including squamous cell carcinoma (squamous cell carcinoma) in 45 cases, adenocarcinoma in 9 cases, and small cell undifferentiated carcinoma in 10 cases). Benign lung lesions (Inflammation) group 31 cases. Paraffin-embedded lung specimens were serially sectioned at 5 μm for HE staining and Feulgen staining, respectively. The latter was further tested for DNA content using an internationally-standardized CAS-200 image cytometer (ICM). Analysis indicators include DNA quality, DNA index (DI), proliferation index (PI), percentage of S phase, nuclear area, histogram type, and ploidy status. Results: The nuclear DNA, DI, PI, S% and nuclear area of the lung cancer group were significantly higher than those of the lung inflammation group (P<0.05 or P<0.01). The DNA histogram type of the lung cancer group was mainly type III. Type VI and type VI (79.7%) showed no type I. In contrast, in the lung inflammation group, none of the cases was type III and type VI, mostly type I (90.3%). There were significant differences between the two groups. The difference was statistically significant (P<0.01). There was a significant difference in the nuclear DNA ploidy between the two groups (P<0.01). The lung cancer group was mostly aneuploid and the heteroploid detection rate was 96.9%. The disease group is diploid. Using heteroploid as the standard, combined with the percentage of S phase, the sensitivity of diagnosis of lung cancer was 96.7%, and the specificity was 100%. Conclusion: Use ICM to detect the fiber bronchus?