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目的:探讨绿茶提取物表没食子儿茶素没食子酸酯(EGCG)对结肠癌细胞株LoVo细胞和SW480细胞的作用及其可能的机制。方法:采用不同浓度的EGCG(0、10、20、35μg/mL)对LoVo细胞和SW480细胞进行处理,MTT法检测细胞增殖情况;流式细胞仪检测细胞凋亡和细胞周期变化;实时荧光定量PCR检测细胞HES1与JAG1基因的表达情况。计量资料采用采用t检验,计数资料采用χ2检验。结果 10、20、35μg/mL EGCG对LoVo细胞和SW480细胞增殖均具有抑制作用,抑制率均在EGCG浓度为35μg/mL时达到最高,其抑制作用且呈浓度依赖性,不具有时间依赖性。经0μg/ml ECCG处理后LoVo细胞、SW480细胞凋亡率分别为7.72%,16.43%。经20、35μg/mL ECCG处理后,LoVo细胞和SW480细胞凋亡率分别为15.1%,19.13%和21.4%,40.81%,与0μg/mL ECCG处理后比较,差异有统计学意义((χ2=6.765,19.702,P<0.05)。35μg/mL EGCG能将LoVo细胞和SW480细胞的细胞周期阻滞在GO/G1期,阻碍其向S期转换。35μg/mLEGCG能下调LoVo细胞HES1基因和JAG1基因的表达水平(t=7.686,10.92,P<0.05)。结论:EGCG对体外培养的LoVo细胞和SW480细胞增殖有明显的抑制作用,且能诱导细胞凋亡和影响细胞周期。其作用机制可能与下调HES1及JAG1的基因表达及激活Notch信号转导途径有关。
Objective: To investigate the effect of green tea extract epigallocatechin gallate (EGCG) on colon cancer cell lines LoVo and SW480 and its possible mechanism. Methods: LoVo cells and SW480 cells were treated with different concentrations of EGCG (0, 10, 20, 35 μg / mL). The proliferation of LoVo cells and SW480 cells were detected by MTT assay. Apoptosis and cell cycle were detected by flow cytometry. PCR detection of cell HES1 and JAG1 gene expression. Measurement data using t test, count data using χ2 test. Results EGCG at 10, 20 and 35 μg / mL had inhibitory effects on the proliferation of LoVo cells and SW480 cells. The inhibition rates were all highest at the concentration of 35 μg / mL EGCG. The inhibitory effect was in a concentration-dependent and time-independent manner. After treated with 0μg / ml ECCG, the apoptotic rates of LoVo cells and SW480 cells were 7.72% and 16.43%, respectively. The apoptotic rates of LoVo cells and SW480 cells treated with 20,35 μg / mL ECCG were 15.1%, 19.13% and 21.4%, 40.81%, respectively. Compared with 0 μg / mL ECCG, the apoptotic rates of LoVo and SW480 cells were statistically significant (χ2 = 6.765,19.702, P <0.05) .35μg / mL EGCG could block the cell cycle of LoVo cells and SW480 cells in the GO / G1 phase, hinder the conversion to S phase.35μg / mL EGCG can down-regulate the HES1 gene and JAG1 gene in LoVo cells (T = 7.686,10.92, P <0.05) .Conclusion: EGCG can significantly inhibit the proliferation of LoVo cells and SW480 cells in vitro and induce cell apoptosis and cell cycle, which may be related to Downregulation of HES1 and JAG1 gene expression and activation of Notch signal transduction pathway.