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目的探讨血管紧张素原(AGT)T704C rs699位点多态性与小动脉粥样硬化性脑梗死(SA)发病机制的关系。方法以SA组186例和对照组94例为研究对象,采用SNaPshot技术及GeneMapper4.0软件分析AGT T704C rs699位点的基因分型情况,比较C(T)基因特征及等位基因频率,并进一步采用多元logistic回归,分析脑血管病常见危险因素与所携带基因分型在SA发病中的交互作用。结果 AGTT704C rs699位点在SA组中以携带TT和TC基因型多见(30.1%、41.9%),但患病率以CC基因型为高(80.0%),与TT和TC基因型(65.1%、60.4%)比较,差异均有统计学意义(χ~2=5.24、6.78,均P<0.05);SA组T等位基因频率高于C等位基因频率,但差异无统计学意义(χ~2=1.23,P>0.05)。携带AGT CC基因型(OR=3.34,95%CI 1.54~8.43)、高血压病史(OR=5.59,95%CI 2.35~22.61)、老龄(≥70岁)(OR=2.82,95%CI 1.13~10.63)是SA发病的独立危险因素。结论 AGT T704C基因多态性可能是SA发病机制的遗传因素,且携带AGTCC基因型,高血压病史和老年患者具有协同致病效应。
Objective To investigate the association of T704C rs699 polymorphism of angiotensinogen (AGT) with the pathogenesis of atherosclerosis cerebral infarction (SA). Methods The genotypes of AGT T704C rs699 locus were analyzed by SNaPshot technique and GeneMapper4.0 software in 186 cases of SA group and 94 cases of control group. The C (T) gene characteristics and allele frequencies were compared and further analyzed Multivariate logistic regression was used to analyze the interaction between common risk factors of cerebrovascular disease and the genotypes carried by them in the pathogenesis of SA. Results The rs699 locus of AGTT704C was more common in the SA group than in the TT and TC genotypes (30.1%, 41.9%) but the CC genotype was high (80.0% , 60.4% respectively), the difference was statistically significant (χ ~ 2 = 5.24,6.78, all P <0.05). The frequency of T allele in SA group was higher than that in C allele, but the difference was not statistically significant (χ ~ 2 = 1.23, P> 0.05). Patients with AGT CC genotype (OR = 3.34, 95% CI 1.54 ~ 8.43), history of hypertension (OR = 5.59,95% CI 2.35 ~ 22.61), age (≥70 years) 10.63) is an independent risk factor for SA. Conclusion The genetic polymorphism of AGT T704C gene may be the genetic factor of pathogenesis of SA, and it has the synergistic pathogenic effect in AGTCC genotype, hypertension history and elderly patients.