The objective of the whole-spacecraft vibration isolation (WSVI) system is to reduce the launch-induced dynamic loads and the quality control cost of the satellite and its components, and to increase the launch reliability by insertion of isolators between the satellite and the launch vehicle. A niche hybrid genetic algorithm (NHGA) is proposed to optimize stiffness and damping of the isolators. Through the comparison of the frequency response analysis results, it shows that the optimized WSVI system more effectively reduces spacecraft axial / lateral response due to the broadband structure-born launch environment. At the same time, the case of the whole-spacecraft vibration isolation optimization design demonstrates the efficiency and validity of the genetic algorithm.