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观察高糖对内皮素－１（ＥＴ－１）促兔主动脉血管平滑肌细胞（ＶＳＭＣ）增殖的影响．方法： ＶＳＭＣ分别培养于含正常葡萄糖、高糖或高渗（５．５，２５，葡萄糖 ５．５＋甘露醇 １９．５ ｍｍｏｌ·Ｌ－１）的培养基中［３Ｈ］胸腺嘧啶掺入法检测ＤＮＡ合成速率，蛋白质印迹法检测磷酸化 ｐ４４／４２ ＭＡＰＫ的表达．结果：在 １０至 １０－８ｍｏｌ·Ｌ－１浓度范围内， ＥＴ－１以浓度依赖方式增加 ＶＳＭＣ的［３Ｈ］胸腺嘧啶掺入及磷酸化ｐ４４／４２ ＭＡＰＫ的表达，从 １０－１１到 １０－８ｍｏｌ·Ｌ－１，培养于高糖的 ＶＳＭＣ对相同浓度 ＥＴ－１的增殖反应性高于正常糖或高渗培养条件下的ＶＳＭＣ（Ｐ＜ ０．０５，或 Ｐ＜ ０．０１），而在后两种条件下，ＶＳＭＣ对ＥＴ－１的增殖反应无显著差别．同样，在高糖条件下，ＥＴ－１诱导的ＶＳＭＣ磷酸化ｐ４４／４２ＭＡＰＫ的表达较正常糖和高渗ＶＳＭＣ增加 ６０％－６５％结论：高糖增强ＶＳＭＣ对ＥＴ－１的增殖反应性，可能与磷酸化的 ｐ４４／４２ ＭＡＰＫ高表达有关
To observe the effect of high glucose on the proliferation of aortic vascular smooth muscle cells (VSMC) induced by endothelin-1 (ET-1). METHODS: VSMCs were cultured in culture medium containing normal glucose, high glucose or hypertonic (5.5,25, glucose 5.5+ mannitol 19.5 mmol·L-1) [3H] thymidine incorporation DNA synthesis rate and Western blotting were used to detect the expression of phosphorylated p44 / 42 MAPK. Results: ET-1 increased [3H] thymidine incorporation and phosphorylated p44 / 42 MAPK expression in VSMCs in a concentration-dependent manner from 10-11 to 10- 8mol·L-1 VSMCs cultured in high glucose had the same higher proliferative reactivity to ET-1 than those cultured in normal glucose or hypertonic medium (P <0.05 or P <0.01) In the latter two conditions, VSMC proliferative response to ET-1 no significant difference. Similarly, ET-1-induced VSMC phosphorylation of p44 / 42MAPK increased by 60% -65% compared with normal and hypertonic VSMCs. CONCLUSION: Hyperglycemia increases the proliferative reactivity of VSMCs to ET-1, It is related to the high expression of phosphorylated p44 / 42 MAPK