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目的:对昆布多糖进行不同硫取代度的硫酸酯化修饰,并对其产物的硫酸基含量、糖含量与分子量进行检测,为研究不同硫取代度昆布多糖硫酸酯的生物活性奠定物质基础。方法:采用氯磺酸-吡啶法对昆布多糖进行硫酸化修饰,通过改变硫酸化修饰条件,来制取不同硫酸基取代度的昆布多糖硫酸酯;利用盐酸水解-硫酸钡比浊法测定昆布多糖硫酸酯的硫酸基含量,并通过公式求得其硫取代度;用苯酚-硫酸法测定昆布多糖硫酸酯的多糖含量,并使用HPGPC法测定其分子量。结果:两种不同硫取代度昆布多糖硫酸酯的硫酸基含量分别为37.8%、45.92%,取代度分别为1.07、1.51,糖含量分别为44.52%、37.19%,分子量分别为13000、16000。结论:利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化修饰,该方法可以获取不同取代度产物,酯化率高。
OBJECTIVE: To sulphate modification of laminaran with different sulfur substitution, and to detect the content of sulfate group, sugar content and molecular weight of lanthanum polysaccharides, which laid the material foundation for studying the biological activity of laminarin with different sulfur substitution. Methods: Kraft polysaccharides were sulfated with chlorosulfonic acid-pyridine method. Laminar polysulfate with different degree of substitution of sulfate was prepared by changing the conditions of sulfation modification. The content of laminarin was determined by hydrochloric acid hydrolysis-barium sulfate turbidimetry Sulfuric acid ester content, and through the formula to obtain the degree of sulfur substitution; Determination of polysaccharide sulphate polysaccharide content with phenol - sulfuric acid method, and determination of the molecular weight using HPGPC method. Results: The content of sulfate groups in two different sulfur-substituted laminns was 37.8% and 45.92%, respectively, and the degrees of substitution were 1.07 and 1.51 respectively. The contents of carbohydrates were 44.52% and 37.19%, respectively, and the molecular weights were 13000 and 16000 respectively. Conclusion: Sulfated esterification of laminarin with chlorosulfonic acid-pyridine method can obtain products with different degree of substitution and high esterification rate.