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Ｉ－６９杨插穗分别培养在含有不同浓度Ｃｅ（ＮＯ３）３的营养液中，经快速冷冻、冷冻干燥、无水塑料包埋及干刀薄切片法，用Ｘ－射线能谱微区分析测定Ｃｅ及其它无机离子在茎尖分生组织和叶的不同组织中细胞壁、细胞质、细胞核及叶绿体中的含量及分布。在１０ｐｐｍＣｅ（ＮＯ３）３处理下，茎尖中Ｃｅ的含量比对照组和１００ｐｐｍＣｅ（ＮＯ３）３处理组高出近１倍，叶的亚细胞中的Ｋ、Ｐ、Ｓ、Ｆｅ的含量也呈增加的趋势。特别是Ｍｇ的含量增加显著，在保卫细胞的细胞质中Ｍｇ的含量比对照组高出３．５倍，而在表皮细胞的细胞核和液泡中的含量则比对照组高出近１３倍。但在１００ｐｐｍＣｅ（ＮＯ３）３处理下，无机离子在叶中亚细胞微区间的含量则显著下降
I-69 Yang cuttings were cultured in nutrient solution containing different concentrations of Ce (NO3) 3, by rapid freezing, freeze-drying, anhydrous plastic entrapment and dry knife thin section method, X-ray spectroscopy microanalysis Contents and distribution of Ce and other inorganic ions in cell wall, cytoplasm, nucleus and chloroplast in different tissues of shoot apical meristem and leaf. Under 10ppm Ce (NO3) 3 treatment, the content of Ce in the shoot tip was almost double that of the control group and the 100ppm Ce (NO3) 3 treatment group, and the content of K, P, S and Fe in the leaf subcellular was also increased the trend of. Particularly, the content of Mg increased remarkably. The content of Mg in the cytoplasm of the guard cells was 3.5 times higher than that in the control group, and was nearly 13 times higher in the nuclei and vacuoles of the epidermal cells than in the control group. However, under the treatment of 100ppmCe (NO3) 3, the content of inorganic ions in the subcellular micro-compartments of leaves significantly decreased