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目的研究线粒体DNAD-环区在胃癌细胞中的突变对细胞内活性氧水平及细胞周期的影响。方法采用PCR方法对20例胃癌组织及其邻近的正常胃黏膜线粒体DNAD-环区进行扩增并测序,根据测序结果分为突变组和无突变组;用流式细胞仪测定两组活性氧及细胞周期,以比较两组中活性氧及细胞周期的差异。结果在20例癌组织中,有7例(35%)存在线粒体DNAD-环区突变,突变位点18个,其中4个属微卫星不稳定。突变组中的活性氧、细胞增殖及凋亡率较无突变组均显著增高(P<0.05)。结论胃癌细胞线粒体DNAD-环区是一个具有高度突变性的区域。线粒体DNAD-环区的突变可能在胃癌的发生、发展过程中起着重要的作用。
Objective To investigate the effects of mutations in mitochondrial DNA D-loop region in gastric cancer cells on intracellular reactive oxygen species (ROS) and cell cycle. Methods The mitochondrial DNA-loop region of 20 cases of gastric cancer and its adjacent normal gastric mucosa were amplified by PCR and sequenced. The mitochondrial DNA-loop regions were divided into mutated group and non-mutated group by flow cytometry. The levels of reactive oxygen species Cell cycle to compare reactive oxygen species and cell cycle differences between the two groups. Results In 20 cases of cancerous tissues, mitochondrial DNA D-loop mutations were found in 7 of 35 cases (18%), of which 18 were mutated. Four of them were microsatellite instable. The levels of reactive oxygen species, cell proliferation and apoptosis in the mutant group were significantly higher than those in the non-mutation group (P <0.05). Conclusion The mitochondrial DNA D-loop region of gastric cancer cells is a highly mutated region. Mutations in the mitochondrial DNA D-loop region may play an important role in the occurrence and development of gastric cancer.