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利用核黄素与光作用产生Ｏ２，Ｆｅｎｔｏｎ反应产生ＯＨ·，以及Ｈ２Ｏ２引起的红细胞氧化溶血的方法，研究了丹皮总甙（ＴＧＭ）和白芍总甙（ＴＧＰ）对Ｏ２及ＯＨ·的清除作用和对溶血反应的影响。结果ＴＧＭ和ＴＧＰ对Ｏ２产生５０％清除率时的浓度分别为２．３１ｍｇ／Ｌ和２．６４ｍｇ／Ｌ，对ＯＨ·产生５０％清除率时的浓度分别为２５．６９ｍｇ／Ｌ和１１７．９６ｍｇ／Ｌ，抑制Ｈ２Ｏ２引起溶血反应的ＩＣ５０分别是０．９４ｍｇ／Ｌ和１．４１ｍｇ／Ｌ。结果表明ＴＧＭ和ＴＧＰ对自由基有清除作用，并能抑制Ｈ２Ｏ２引起的溶血反应，且ＴＧＭ的作用强于ＴＧＰ。
The removal of O2 and OH· by TGM and TGP was studied by using riboflavin to generate O2 with light, Fenton reaction to produce OH·, and H2O2 induced oxidative hemolysis of red blood cells. Effects and effects on hemolytic reactions. Results The concentrations of TGM and TGP for 50% clearance of O2 were 2.31 mg/L and 2.64 mg/L, respectively. For 50% clearance of OH·, the concentrations were 25.69 mg/L and 117.96 mg, respectively. /L, IC50 for inhibition of hemolytic reaction induced by H2O2 was 0.94 mg/L and 1.41 mg/L, respectively. The results showed that TGM and TGP had a scavenging effect on free radicals and could inhibit the hemolytic reaction caused by H2O2, and TGM was stronger than TGP.