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目的考察新风胶囊对干燥综合征模型大鼠肺功能的作用,及其对TGF-β1-ERK1信号通路的影响。方法将50只SD大鼠随机分为正常对照组、模型对照组和羟氯喹(HCQ)组、白芍总苷(TGP)组、新风胶囊(XFC)治疗组,每组10只,除正常对照组外,采用完全弗氏佐剂+同种鼠颌下腺抗原诱导方法,向每只大鼠两后足跖部注射与弗氏完全佐剂充分乳化后的颌下腺蛋白混合抗原0.2 mL诱发大鼠干燥综合征模型。用动物肺功能仪检测大鼠肺功能,检测各组大鼠饮水量及体质量的变化、采用免疫组化法ERK1、TGF-β1的表达,采用ELISA法检测血清细胞因子(IL-17、IL-4)的变化。结果与正常对照组(NC)比较,模型对照组(MC)大鼠体质量、血清IL-4明显降低,饮水量、颌下腺/肺指数、颌下腺病理评分、ERK1、TGF-β1积分及IL-17升高(P<0.01或P<0.05),肺功能参数降低(P<0.01或P<0.05);与MC组比较,XFC组体质量、肺功能参数50%肺活量的最大呼气流量(FEF50)、最大呼气中段流量(MMF)升高,IL-4表达升高,饮水量、颌下腺/肺指数、颌下腺病理评分、血清IL-17的表达、ERK1、TGF-β1积分降低(P<0.01或P<0.05)。与HCQ组相比,XFC组体质量、IL-17明显降低(P<0.01),FEF25、FEF75、MMF升高(P<0.01或P<0.05);与TGP组比较,XFC组肺指数、IL-17降低(P<0.01或P<0.05)。结论 SS大鼠存在肺功能下降,可能与TGF-β1-ERK1信号通路活化相关。中药XFC能够下调TGF-β1、抑制ERK1磷酸化、降低免疫炎症反应、改善肺功能。
Objective To investigate the effect of Xinfeng Capsule on lung function in rats with Sjogren's Syndrome and its effect on TGF-β1-ERK1 signaling pathway. Methods Fifty Sprague-Dawley rats were randomly divided into normal control group, model control group and HCQ group, TGP group and XFC group, with 10 rats in each group. Except the normal control group, Group, using complete Freund's adjuvant + allogeneic submandibular gland antigen induction method, each rat was injected with 0.2 mL of submandibular glandular protein mixed antigen fully emulsified with Freund's complete adjuvant to induce rat's dry synthesis Levy model. The lung function of rats was detected by animal lung function test. The changes of drinking water and body weight of rats in each group were detected. The expressions of ERK1 and TGF-β1 were detected by immunohistochemistry. The levels of serum cytokines (IL-17, IL -4) changes. Results Compared with the normal control group, the body weight, serum IL-4 level, water intake, submandibular gland / lung index, submandibular gland pathological score, ERK1, TGF-β1 and IL-17 (P <0.01 or P <0.05). Compared with MC group, body mass and maximum expiratory flow (FEF50) of 50% (P <0.01 or P <0.01 or P <0.01 or P <0.01 or P <0.01 or P <0.01 or P <0.01 or P <0.01 or P < P <0.05). Compared with the HCG group, the body mass, IL-17 and the FEF25, FEF75 and MMF in the XFC group were significantly decreased (P <0.01 or P <0.05) -17 (P <0.01 or P <0.05). Conclusion The pulmonary function decline of SS rats may be related to the activation of TGF-β1-ERK1 signaling pathway. Chinese medicine XFC can down-regulate TGF-β1, inhibit ERK1 phosphorylation, reduce immune inflammation and improve lung function.