论文部分内容阅读
To investigate the gene expression at transcription level of growth factor Wnt 5A in different phase during the cell cycle Methods We synchronized the renal cell carcinoma GRC 1 cell line by double thymidine blocks and high pressure N 2O gae methods and amplified Wnt 5A cDNAs from different phase using Semi quantitative RT PCR (reverse transcriptase polymerase chain reaction) The PCR products were electrophoresised on the agrose gel and detected by Gel Doc 1000 computer controlled system integrating the volumes of each band, representing the intensities of all pixels in a defined band Results The different mRNA expressions of growth factor Wnt 5A was detected in RCC GRC 1 cell line In S phase, the highest level of Wnt 5A transcript was observed, and in G1 and M phase, medial and lowest, respectively The differences between S and M stages were statistically significant ( P <0 05) Conclusion Growth factor Wnt 5A has the potential effect on tumorigenesis It contributes to all phases during cell cycle but in S phase especially
To investigate the gene expression at transcription level of growth factor Wnt 5A in different phase during the cell cycle Methods We synchronized the renal cell carcinoma GRC 1 cell line by double thymidine blocks and high pressure N 2O gae methods and amplified Wnt 5A cDNAs from different phase using Semi quantitative RT PCR (reverse transcriptase polymerase chain reaction) The PCR products were electrophoresised on the agrose gel and detected by Gel Doc 1000 computer controlled system integrating the volumes of each band, representing the intensities of all pixels in a defined band Results The different mRNA expressions of growth factor Wnt 5A was detected in RCC GRC 1 cell line In S phase, the highest level of Wnt 5A transcript was observed, and in G1 and M phase, medial and lowest, respectively The differences between S and M are statistically significant Significant (P <0 05) Conclusion Growth factor Wnt 5A has the potential effect on tumorigenesis It contributes to all phases during cell cycle but in S phase especially