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目的探讨不孕不育妇女宫颈分泌物中解脲脲原体(Uu)的感染状况及与抗精子抗体(AsAb)的相关性。方法应用荧光定量PCR法和液体培养检测101例不孕不育患者宫颈分泌物,采用ELISA检测血清中AsAb。结果荧光定量PCR法检出Uu阳性64例(63.36%),液体培养法检出47例(46.53%),荧光定量PCR法检出的阳性率明显高于液体培养法(P<0.05);64例Uu阳性患者检测出AsAb51例,37阴性患者检测出AsAb 8例,Uu阳性组AsAb阳性率高于Uu阴性组(P<0.05)。结论女性不孕不育与Uu感染密切相关,PCR法诊断不孕不育Uu感染更有价值,且AsAb的产生与Uu感染有关。
Objective To investigate the relationship between Uu infection and anti-sperm antibody (AsAb) in cervical secretions of infertile women. Methods The cervical secretions of 101 infertile women were detected by fluorescence quantitative PCR and liquid culture. Serum AsAb was detected by ELISA. Results The positive rate of Uu was 64.3% (63.36%) by fluorescence quantitative PCR, 47 (46.53%) were detected by liquid culture, and the positive rate by fluorescence quantitative PCR was significantly higher than that by liquid culture (P <0.05). Asu positive cases were detected in 51 cases of Asu, AsAb detected in 37 negative patients 8 cases, Uu positive group AsAb positive rate was higher than Uu negative group (P <0.05). Conclusion Female infertility is closely related to Uu infection. PCR is more valuable for the diagnosis of Uu infertility, and AsAb production is associated with Uu infection.