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在室内用3种不同棉酚含量的棉花饲养甜菜夜蛾幼虫,并在不同取食时间测定了甜菜夜蛾幼虫的营养物质、消化酶、保护酶和解毒酶活性的变化.实验结果表明,甜菜夜蛾幼虫取食高棉酚含量的棉花M9101品种6h后,体内的游离脂肪酸较取食其他两种低酚含量棉花出现了显著性的降低.甜菜夜蛾幼虫取食高棉酚含量的棉花M9101品种24h后,体内的胰蛋白酶较取食1,4,6h后出现了显著性的增加.甜菜夜蛾幼虫取食高棉酚含量的棉花M9101品种1,4,6,24h后,体内的过氧化氢酶和总超氧化物歧化酶显著高于取食低酚棉花品种ZMS13和HZ401,但体内显著较低的羧酸酯酶和乙酰胆碱酯酶酶活性显著低于取食低酚棉花品种ZMS13和HZ401.多因素方差分析表明,不同棉酚含量棉花品种除对甜菜夜蛾幼虫体内淀粉酶活性无影响外,对其他消化酶、保护酶和解毒酶活性均有显著性的影响.棉花品种和甜菜夜蛾不同为害时间的交互作用可显著影响甜菜夜蛾幼虫体内的羧酸酯酶和过氧化氢酶活性.测定对甜菜夜蛾不同为害时间体内酶活性对不同棉酚含量的棉花的响应,可有效评估植食性昆虫在植物次生代谢物质压力下的生理反应和抗性变化.
The larvae of beet armyworm were fed with three kinds of cotton with different gossypol contents, and the changes of nutrient, digestive enzymes, protective enzymes and detoxification enzymes of beet armyworm larvae were determined at different feeding times.The experimental results show that the sugar beets Cotton bollworm larvae fed cotton gossypol content of M9101 6h after the free fatty acids in the body to eat the other two low-phenol content of cotton showed a significant reduction of beet armyworm larvae fed cotton gossypol content M9101 After 24 hours, the trypsin in vivo showed a significant increase after 1, 4 and 6 hours of feeding.With the increase of gossypol content in cotton M9101 cultivars, Catalase and total superoxide dismutase (SOD) were significantly higher than those of ZMS13 and HZ401, but significantly lower in vivo carboxylesterase and AChE activities were significantly lower than those of ZMS13 and ZMS13 HZ401. Multivariate analysis of variance showed that cotton varieties with different gossypol content had significant effects on the activity of other digestive enzymes, protective enzymes and detoxification enzymes in addition to the activity of amylase in beet armyworm larvae. Night moth Interaction with damage time could significantly affect the activity of carboxylesterase and catalase in beet armyworm larvae.Determination of the enzyme activity in different stages of damage to beet armyworm could respond to cotton with different gossypol content, PHYSIOLOGICAL RESPONSES AND RESISTANCE CHANGES OF PLANT SPECIES UNDER PRESSURE OF PLANT SECONDARY METABOLITES.