极低密度脂蛋白(VLDL)与小鼠腹腔巨噬细胞温育24小时和48小时后,其硫代巴比妥酸反应物质值明显高于无细胞对照组,琼脂糖电泳速度也加快,两者一致。结果说明小鼠腹腔巨噬细胞可以氧化修饰极低密度脂蛋白。VLDL在体外用Cu~(2+)修饰后。硫代巴比妥酸反应物质明显增加,琼脂糖凝股电泳速度加快。比较正常的VLDL(n—VLDL)及Cu~(2+)氧化的VLDL(Ox—VLDL)对内皮细胞粘附单核细胞的影响时,发现在所用各种浓度下,Ox—VLDL单核细胞的粘附率比n—VLDL组大得多(P<0.0001),说明VLDL被氧化后明显增加单核细胞与内皮细胞的粘附。 Very low density lipoprotein (VLDL) and mouse peritoneal macrophages after 24 hours and 48 hours after incubation, the thiobarbituric acid reaction substance value was significantly higher than the cell-free control group, agarose electrophoresis speed also accelerated, two Consistent. The results show that mouse peritoneal macrophages can oxidize very low density lipoprotein. VLDL is modified with Cu 2+ in vitro. Thiobarbituric acid reaction substances increased significantly agarose gel electrophoresis speed. When comparing the effect of normal VLDL (n-VLDL) and Cu 2+ oxidized VLDL (Ox-VLDL) on endothelial cell adhesion to monocytes, it was found that at various concentrations used Ox-VLDL monocytes (P <0.0001), indicating that VLDL oxidized significantly increased the adhesion of monocytes to endothelial cells.