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[目的]了解高羊茅SRP基因结构与功能以及在多种逆境胁迫下的表达变化。[方法]以高羊茅转录组学测序获得的SRP序列为基础,从高羊茅叶片c DNA中应用3’RACE和5’RACE方法扩增出SRP基因全长c DNA序列。[结果]SRP基因c DNA全长为1 165bp,具有一个完整的长度为855 bp的开放阅读框,编码蛋白为284个氨基酸,包含一个REF结构域。通过生物信息学的方法预测SRP基因的结构及功能发现,Fa SRP与单子叶植物SRP蛋白具有相对较高的序列同源性。应用荧光定量PCR技术分析了SRP基因在低氮、干旱、高热以及高盐逆境胁迫下的表达变化发现SRP基因能够应答低氮、干旱、高温等胁迫,但应答机制不一样,可能是通过不同途径调节植物抗逆性,Fa SRP对高盐胁迫不敏感。[结论]该研究为培育抗旱、耐热、营养高效的高羊茅品种提供候选基因与技术储备。
[Objective] The research aimed to understand the structure and function of SRP gene of Tall Fescue and its expression changes under various stress conditions. [Method] Based on the SRP sequence obtained from the transcriptomic sequencing of tall fescue, the full length cDNA of SRP gene was amplified by 3’RACE and 5’RACE from the c DNA of tall fescue leaves. [Result] The full length cDNA of SRP gene was 1 165 bp with a complete open reading frame of 855 bp. The encoded protein was 284 amino acids and contained a REF domain. Prediction of the structure and function of SRP gene by bioinformatics method showed that Fa SRP has relatively high sequence homology with monocotyledonous plant SRP protein. The changes of SRP gene expression under low nitrogen, drought, high heat stress and high salt stress stress were analyzed by fluorescence quantitative PCR. SRP gene was able to respond to low nitrogen, drought and high temperature stress, but the response mechanism was different. It may be through different ways Regulating plant stress resistance, Fa SRP is insensitive to high salt stress. [Conclusion] The research provided the candidate gene and technical reserve for fostering the tall fescue varieties resistant to drought, heat, nutrition and high efficiency.