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研究哮喘患者肺泡巨噬细胞 (AM)源性一氧化氮 (NO)、内皮素 (ET)的变化及硝酸甘油 (NTG)、地塞米松(DXM)对两者的影响及机制。对 15例轻、中度过敏性支气管哮喘发作期患者的AM(分为未干预组、DXM干预组、NTG干预组 ) ,7名健康自愿受试者的AM(未干预组 )培养 48h ,用镀铜镉还原法、放射免疫法和原位杂交法分别测定AM培养上清液中NO ,ET水平和iNOS mRNA ,ET mRNA的表达。结果发现 ,哮喘AMiNOS mRNA ,ET mRNA表达增强 ,分别导致NO ,ET水平升高 ;NTG以直接作用的方式促进AM源性NO的产生 ,反馈抑制iNOS mRNA表达并明显抑制ETmRNA的表达 ,降低ET的水平 ;DXM降低哮喘AM源性NO ,ET水平及iNOS mRNA ,ET mRNA的表达 ,尤以抑制iNOS mRNA表达和降低NO水平为甚 ,使NO ,ET处于低水平的异常状态。
To investigate the effects of nitric oxide (NTG) and dexamethasone (DXM) on the changes of nitric oxide (NO), endothelin (ET) and alveolar macrophages (AM) Fifteen patients with mild to moderate allergic bronchial asthma were divided into AM group (no intervention group, DXM intervention group and NTG intervention group) and AM (no intervention group) of seven healthy volunteers for 48 hours. The levels of NO, ET and the expression of iNOS mRNA and ET mRNA in AM culture supernatants were determined by copper-cadmium reduction, radioimmunoassay and in situ hybridization. The results showed that the expression of AMINOS mRNA and ET mRNA in asthma increased, which led to the increase of NO and ET, respectively. NTG promoted the production of AM-derived NO by direct action, inhibited the expression of iNOS mRNA and significantly inhibited the expression of ET mRNA, DXM decreased the expression of AM, NO, ET and iNOS mRNA and ET mRNA in asthmatic rats, especially in inhibiting the expression of iNOS mRNA and decreasing the level of NO.