通过测定急性和慢性乙型肝炎患者肝内细胞趋化因子受体(CCR5)和CXCR3水平,结合患者血中乙型肝炎病毒(HBV)含量,探讨趋化因子受体在乙型肝炎发病及其转归中的作用。1.材料与方法:(1)试剂:链霉素抗生物素蛋白过氧化酶免疫染色超敏试剂盒,兔抗CCR5和CXCR3单克隆抗体,抗体效价1:500.均购自深圳华氏试剂公司,按试剂盒说明书进行操作。(2)病例:大连市第六人民医院2002年1月至2003年 By determining the levels of chemokine receptor CCR5 and CXCR3 in patients with acute and chronic hepatitis B and the level of hepatitis B virus (HBV) in the blood of patients, we explored the role of chemokine receptors in the pathogenesis of hepatitis B and its The role of the outcome. 1. Materials and Methods: (1) Reagents: streptavidin-peroxidase immunostaining kit, rabbit anti-CCR5 and CXCR3 monoclonal antibody, antibody titer 1: 500. were purchased from Shenzhen Fahrenheit reagent Company, according to kit instructions. (2) Case: Sixth People’s Hospital of Dalian from January 2002 to 2003