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目的:探讨补肾活血汤对腰椎间盘退变大鼠TNF-α表达的影响,为延缓或阻止椎间盘退变提供客观评价指标。方法:将72只大鼠随机分为空白组、模型组、中药组,每组24只,采用皮下注射D-半乳糖法建立衰老大鼠椎间盘退变模型,造模成功后中药组予补肾活血汤灌胃,空白组与模型组予蒸馏水灌胃。连续给药4周后以Elisa法检测大鼠椎间盘组织中TNF-α的含量,以光密度OD值表示。结果:与空白组比较,模型组TNF-α含量明显升高,差异有统计学意义(P<0.05);中药组TNF-α含量低于模型组,两组比较,差异有统计学意义(P<0.05)。结论:补肾活血汤可能是通过抑制大鼠退变腰椎间盘组织中TNF-α的表达来减缓椎间盘的退变过程。
Objective: To investigate the effect of Bushen Huoxue Decoction on the expression of TNF-α in rats with lumbar disc degeneration and provide an objective evaluation index for delaying or preventing the degeneration of intervertebral disc. Methods: Seventy-two rats were randomly divided into blank group, model group and traditional Chinese medicine group, 24 rats in each group. Subcutaneous injection of D-galactose was used to establish the model of disc degeneration in aging rats. After the model was established, Gavage the soup, the blank group and model group was given distilled water. Four weeks after continuous administration, the contents of TNF-α in the rat intervertebral disc tissue were detected by Elisa method and expressed as optical density OD value. Results: Compared with the blank group, the content of TNF-α in the model group increased significantly (P <0.05), while the content of TNF-α in the Chinese herbal medicine group was lower than that of the model group (P <0.05). The difference was statistically significant <0.05). Conclusion: Bushen Huoxue Decoction may slow down the process of disc degeneration by inhibiting the expression of TNF-α in degenerative lumbar disc tissue of rats.